| 论文题目： | Rat Embryonic Stem Cells Produce Fertile Offspring Through Tetraploid Complementation |
| 论文题目英文： | |
| 作者： | 李天达①，冯桂海①，李宇飞①，汪妹①，毛俊杰，王加强，李鑫，王雪鹏，曲彬，王乐韵，Xin-Xin Zhang，万海峰，Tong-Tong Cui，Cong Wan，刘蕾，#赵小阳，胡宝洋，李伟，周琪* |
| 论文出处： | |
| 年： | 2017 |
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| 期： | DOI: 10.1073/pnas.1708710114 |
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| 联系作者： | 周琪 |
| 发表期刊： | Proceedings of the National Academy of Sciences |
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| 论文连接 | http://www.pnas.org/content/early/2017/10/20/1708710114.full |
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| 摘要: | Pluripotency of embryonic stem cells (ESCs) can be functionally assessed according to the developmental potency. Tetraploid complementation, through which an entire organism is produced from the pluripotent donor cells, is taken as the most stringent test for pluripotency. It remains unclear whether ESCs of other species besides mice can pass this test. Here we show that the rat ESCs derived under 2i (two small molecule inhibitors) conditions at very early passages are able to produce fertile offspring by tetraploid complementation. However, they lose this capacity rapidly during culture due to a nearly complete loss of genomic imprinting. Our findings support that the naïve ground state pluripotency can be captured in rat ESCs but also point to the species-specific differences in its regulation and maintenance, which have implications for the derivation and application of naïve pluripotent stem cells in other species including human. |
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