郭靖涛，中国科学院动物研究所研究员，器官再生与智造全国重点实验室副主任。

2013年本科毕业于北京大学，后赴美国犹他大学攻读博士，毕业后在犹他大学医学院继续深造，进行博士后研究。2020年7月受聘于犹他大学医学院，任准聘助理教授，组建独立课题组，并获多个NIH R01基金资助。2021年12月加入中国科学院动物研究所干细胞与生殖生物学国家重点实验室，任研究组组长。2022年入选国家海外高层次人才计划；获中国科学院战略先导项目、国家重点研发计划、国家自然科学基金面上项目等多个国家级项目资助。以第一作者或通讯作者（含共同）在Cell、 Cell Stem Cell、 Nature Cell Biology、 Nature Aging、 Nature Communications、 Developmental Cell、 Cell Research等期刊上发表研究成果,并受邀在中国、美国、德国、瑞典等多个研究所和高校分享研究成果。长期担任Cell Stem Cell、 Nature Aging、 Nature Communications、 Cell Reports Medicine等期刊独立审稿人。

1. 生殖细胞与性腺发育的调控研究

聚焦人类精原干细胞的发育与分化调控，发现了一类人类特有的精原干细胞亚型，提出其双向可逆的发育模型，为精原干细胞的体外培养与定向分化提供了关键靶标分子；系统解析了从早期胚胎至青春期及成年期睾丸发育的核心事件，阐明了精原干细胞完整建立的过程，揭示了生殖细胞与睾丸微环境中体细胞的协同发育规律，为优化青少年肿瘤患者生育力保存技术提供了新思路。

2.体外疾病模型构建与机制解析

针对睾酮缺乏、男性不育及相关代谢疾病的机制研究面临的核心障碍——缺乏能够模拟睾丸复杂微环境与动态内分泌过程的体外模型，从发育原理出发，整合器官芯片与细胞工程技术，构建仿生睾丸类器官芯片平台；通过构建生理及病理状态下睾丸微环境的体外模型，深入揭示男性不育的细胞与分子机制。研究发现，隐睾症患者的精子发生障碍主要源于精原干细胞受损及睾丸内生精微环境的功能异常，尤其是肥大细胞数量增多与脱颗粒激活等免疫相关事件，为未来临床开展精准诊断与靶向治疗奠定了理论基础。

3.人工智能驱动的医学精准决策

通过构建人类早期胚胎3D数字模型，解析了原始生殖细胞的定位、起源、迁移路径及性腺原基建立等关键发育节点的分子轨迹，对理解早期发育异常疾病的发病机制有临床意义；基于大规模多中心临床数据，整合机器学习算法，开发了面向非梗阻性无精子症（NOA）患者显微取精手术预后预测工具SpermFinder（https://huggingface.co/spaces/SpermFinder/TESE）。该在线模型可有效评估精子获取成功率，为临床术前评估与患者知情决策提供智能化支持。

（^#共同第一作者，^*共同通讯作者）

1. Zhang W^#, Hong K^#, Tang Y, Cui L, Lu X, Cheng J, Fang Y, Jiang Q, Zhuang Z, Gao S, Jiang H, Liu Q*, Guo J*, Zhang Z*, Wang X*. Endothelial PDGF signaling dysregulation impairs testicular interstitial homeostasis in diabetes. Advanced Science. 2026 Jan 28.
2. Cui L^#, Lu X^#, Cai H, Zhuang Z, Guo Y, Jiang Q, Zhang Z*, Jiang H*, Wang X*, Guo J*. SPARK: A webserver for comprehensive search and analysis of testicular single-cell transcriptomic data covering full lifecycle and pathology. iMetaOmics. 2026 Jan 10:e70073.
3. Zhang B^#, Zhao X^#, Cao J, Liu Z, Wang X, Li R, Bo H, Xu K*, Guo J*. Single-cell atlas of human penile corpus cavernosum reveals cellular and functional heterogeneity of aging-related erectile dysfunction. Front Endocrinol (Lausanne). 2025 Oct 29;16:1671482.
4. Xi Y^#, Zhang B^#, Zhang Y^#, Zhao L, Liu D, Mao J, Tang W, Zhang H, Lin H, Wang X, Ren P, Tang Y, Yang Y, Hong K*, Guo J*, Zhang Z*, Jiang H*. Machine learning-based personalized prediction of sperm retrieval in patients with non-obstructive azoospermia prior to microdissection testicular sperm extraction: A multi-center cohort study. Andrology. 2025 Sep 8.
5. Ba Omar H^#, Stevens J^#, Haavisto A, Cui Y, Harteveld F, Yang Y, Bjarnason R, Romerius P, Sundin M, Nyström UN, Langenskiöld C, Vogt H, Frisk P, Vepsäläinen K, Petersen C, Cui L, Guo J, Jahnukainen K*, Stukenborg JB*. Early transcriptional states of spermatogonia and marker expressions in the prepubertal human testis following chemotherapy-induced depletion. Hum Reprod. 2025 Aug 1;40(8):1467-1475.
6. Yuan Y^#, Wang X^#, Yan X^#, He N^#, Lu X^#, Yang J^#, Xie X^#, Yuan H, Chen N, Liu Y, Ren H, Zhang R, Cui L, Ren P, Lin S, Cheng S, Yang X, Guo Y, Li R, Yan T, Guo J*, Xiao Z*, Wei Y*, Yu L*.3D reconstruction of a human Carnegie stage 9 embryo provides a snapshot of early body plan formation. Cell Stem Cell. 2025 Jun 5.
7. Cui L^#, Nie X^#, Guo YX^#, Ren P, Guo YF, Wang X, Li R, Hotaling JM, Cairns BR*, Guo J*. Single-cell transcriptomic atlas of human testis across the reproductive lifespan. Nature Aging. 2025 Apr;5(4):658-674.
8. Jiang Q, Cui L, Nie X, Cai H, Zhang W, Lu X, Guo Y, Hotaling JM, Cairns BR, Wang X*, Guo J*. A single-cell transcriptome atlas characterizes the immune landscape of human testes during aging. Aging Cell. 2025 Mar 6;e70032.
9. Cui L^#, Lin S^#, Yang X^#, Xie X^#, Wang X^#, He N^#, Yang J, Zhang X, Lu X, Yan X, Guo Y, Zhang B, Li R, Miao H, Ji M, Zhang R, Yu L*, Xiao Z*, Wei Y*, Guo J*. Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. Nat Cell Biol. 2025 Feb;27(2):360-369.
10. Wang X^#, Cheng L^#, Lu X^#, Jin H^#, Cui L, Guo Y, Guo J*, Xu EY*. Cross-species comparative single-cell transcriptomics highlights the molecular evolution and genetic basis of male infertility. Cell Rep. 2024 Dec 30;44(1):115118.
11. Wang X^#, Wang Y^#, Wang Y^#, Guo Y^#, Zong R, Hu S, Yue J, Yao J, Han C*, Guo J*, Zhao J*. Single-cell transcriptomic and cross species comparison analyses reveal distinct molecular changes of porcine testes during puberty. Commun Biol. 2024 Nov 9;7(1):1478.
12. Wang X^#, Liu Q^#, Zhuang Z^#, Cheng J, Zhang W, Jiang Q, Guo Y, Li R, Lu X, Cui L, Weng J, Tang Y, Yue J, Gao S, Hong K, Qiao J, Jiang H*, Guo J*, Zhang Z*. Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling. Cell Rep Med. 2024 Sep 17;5(9):101709.
13. Cui Y, Harteveld F, Ba Omar HAM, Yang Y, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Frisk P, Vepsäläinen K, Petersen C, Mitchell RT, Guo J, Alves-Lopes JP, Jahnukainen K, Stukenborg JB. Prior exposure to alkylating agents negatively impacts testicular organoid formation in cells obtained from childhood cancer patients. Hum Reprod Open. 2024 Aug 13;2024(3):hoae049.
14. Xiao Z^#, Cui L^#, Yuan Y^#, He N^#, Xie X, Lin S, Yang X, Zhang X, Shi P, Wei Z, Li Y, Wang H, Wang X*, Wei Y*, Guo J*, Yu L*. 3D reconstruction of a gastrulating human embryo. Cell. 2024 May 23;187(11):2855-2874.e19.
15. Lu X^#, Luo Y^#, Nie X^#, Zhang B, Wang X, Li R, Liu G, Zhou Q, Liu Z, Fan L, Hotaling JM, Zhang Z*, Bo H*, Guo J*. Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment. Nat Commun. 2023 Dec 20;14(1):8462.
16. Wamaitha SE^#, Nie X^#, Pandolfi EC, Wang X, Yang Y, Stukenborg JB, Cairns BR, Guo J*, Clark AT*. Single-cell analysis of the developing human ovary defines distinct insights into ovarian somatic and germline progenitors. Dev Cell. 2023 Oct 23;58(20):2097-2111.e3.
17. Matilionyte G, Forsyth G, Guo J, Rimmer MP, Hermann BP, Anderson RA, Mitchell RT. Granulocyte-colony stimulating factor does not prevent in vitro cisplatin-induced germ cell reduction in immature human and mouse testis.  BMC Cancer. 2023 Mar 16 ;23(1):251.
18. Nie X, Munyoki SK, Sukhwani M, Schmid N, Missel A, Emery BR, DonorConnect, Stukenborg JB, Mayerhofer A, Orwig KE, Aston KI, Hotaling JM, Cairns BR*, Guo J*. Single-Cell Analysis of Human Testis Aging and Correlation with Elevated Body Mass Index. Dev Cell. 2022 May 9;57(9):1160-1176.e5.
19. Wang X, Cairns BR*, Guo J*. When spermatogenesis meets human aging and elevated body mass. Life Med. 2022 Jul 9;1(3):267-269.
20. Mahyari E^#, Guo J^#*, Lima AC, Lewinsohn DP, Stendahl AM, Vigh-Conrad KA, Nie X, Nagirnaja L, Rockweiler NB, Carrell DT, Hotaling JM, Aston KI, Conrad DF*. Comparative single-cell analysis of biopsies clarifies pathogenic mechanisms in Klinefelter syndrome. Am J Hum Genet. 2021 Oct 7;108(10):1924-1945.
21. Patel DP, Punjani N, Guo J, Alukal JP, Li PS, Hotaling JM*. The impact of SARS-CoV-2 and COVID-19 on male reproduction and men's health. Fertil Steril. 2021 Apr;115(4):813-823.
22. Guo J*, Sosa E, Chitiashvili T, Nie X, Rojas EJ, Oliver E; DonorConnect; Plath K, Hotaling JM, Stukenborg JB, Clark AT*, Cairns BR*. Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment. Cell Stem Cell. 2021 Apr 1;28(4):764-778.e4.
23. Tharmalingam MD, Matilionyte G, Wallace WHB, Stukenborg JB, Jahnukainen K, Oliver E, Goriely A, Lane S, Guo J, Cairns B, Jorgensen A, Allen CM, Lopes F, Anderson RA, Spears N, Mitchell RT. Cisplatin and carboplatin result in similar gonadotoxicity in immature human testis with implications for fertility preservation in childhood cancer. BMC Med. 2020 Dec 4 ;18(1):374.
24. Murphy PJ^#*, Guo J^#, Jenkins TG, James ER, Hoidal JR, Huecksteadt T, Broberg DS, Hotaling JM, Alonso DF, Carrell DT, Cairns BR*, Aston KI*. NRF2 loss recapitulates heritable impacts of paternal cigarette smoke exposure. PLoS Genet. 2020 Jun 10;16(6):e1008756.
25. Guo J, Nie X, Giebler M, Mlcochova H, Wang Y, Grow EJ; DonorConnect; Kim R, Tharmalingam M, Matilionyte G, Lindskog C, Carrell DT, Mitchell RT, Goriely A, Hotaling JM, Cairns BR*. The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty. Cell Stem Cell. 2020 Feb 6;26(2):262-276.e4.
26. Guo J*, Cairns BR. Isolation and Enrichment of Spermatogonial Stem Cells From Human Testis Tissues. Curr Protoc Stem Cell Biol. 2019 Jun;49(1):e77.
27. Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, Kim R, Carrell DT, Goriely A, Hotaling JM, Cairns BR*. The adult human testis transcriptional cell atlas. Cell Res. 2018 Dec;28(12):1141-1157.
28. Guo J, Grow EJ, Yi C, Mlcochova H, Maher GJ, Lindskog C, Murphy PJ, Wike CL, Carrell DT, Goriely A, Hotaling JM, Cairns BR*. Chromatin and Single-Cell RNA-Seq Profiling Reveal Dynamic Signaling and Metabolic Transitions during Human Spermatogonial Stem Cell Development. Cell Stem Cell. 2017 Oct 5;21(4):533-546.e6.